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Flow Cytometry Shared Resource. BD Fortessa Configuration. KCRB. Room 4161. SN: H64717700057.

3. Depressurize the sheath container by pulling up on the vent valve. 4. Remove the sheath container lid. 5.

tidigare var belagda med kollagen typ I (BD Biosciences, Le Pont de Claix, Frankrike), Cells were washed and run on a LSR- Fortessa (BD Biosciences). Subsequent data were analysed with FloJo X analysis software (FreeStar Ashland, OR, USA). Fortessa flatware embodies a passion for design combined with a commitment to the finest materials.

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RUO (2) Brand. BD LSRFortessa™ (2) Sort by: Instrument Systems (2 of 2) results found. BD LSRFortessa™ BD LSRFortessa Cell BD FACS LSR Fortessa User Manual Flow Cytometry Unit – Biomedical Core facility Technion Faculty of Medicine For help please call: Amir Grau 054-210-0778 Yaakov Sakoury 050-749-8565 Ofer Shenker 052-456-8846 A. Starting Up 1. Turn on the instrument: a.

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BD Fortessa X-20 Log in to BD FACSDiva software. MACHINE SETUP: • Turn on the machine if you are the first user of the day. There is no fluidics startup for the Fortessa. The cytometer disconnects from the BD FACSDiva interface and connects to the CS&T interface.

B 530/30 BB515, FITC, AF488, CFSE, GFP, The BD LSRFortessa Cytometer is a four-laser, 14-parameter special order high- use instrument. It is equipped with 405 nm (violet), 488 nm, (blue), 532 nm There are 4 lasers on Fortessa: blue (488nm),yellow/green (561nm), red (640nm) and a violet (405nm).
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The Fortessas are within rooms that are designated BSL2 space to FORTESSA USER GUIDE COMPUTER SETUP: • Turn on computer before turning on the machine • Windows 10 Username: BDOperator • Password: BDIS#1 • Open Tera Term application on desktop and leave running in background • Log in to BD FACSDiva software MACHINE SETUP: • Turn on the machine if you are the first user of the day. There is no BD FACS LSR Fortessa User Manual Flow Cytometry Unit – Biomedical Core facility Technion Faculty of Medicine For help please call: Amir Grau 054-210-0778 Yaakov Sakoury 050-749-8565 Ofer Shenker 052-456-8846 A. Starting Up 1. Turn on the instrument: a.
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Fortessa FILTERconfig - Lund Stem Cell Center

300mW – 488nm (Blue) solid state 50mW - 561nm (Yellow-Green) solid state 40mW – 640nm (Red) solid state 50mW – 405nm (Violet) solid state. Sample Loading Options. Configuration.

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BD LSR Fortessa.

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BD LSRFortessa™ Cell Analyzer Technical Specifications The BD LSRFortessa™ cell analyzer puts the power of the BD LSR platform into a compact footprint. It can easily fit on the benchtop for more cost-effective space utilization.

Sample loading via tubes. The BD LSR Fortessa is equipped with 5 lasers (355, 405, 488, 561,640 nm). It allows detection of up to 18 parameters simultaneously, 16 fluorescent channels plus forward and side scatter. See configuration. For more information about the LSR Fortessa please visit the Becton Dickinson website or contact dirk.pacholsky@igp.uu.se. FACS Diva quick BD LSRFortessaを用い、健康なドナーの全血をBD Pharm Lyse™ lysing bufferを用いて溶血し、T細胞のサブセットを解析しました。データは、Blue、Red、Violetのレーザーで励起した10カラーのパネルを示します。 BD LSR Fortessa Flow Cytometry Analyzer equipped with HTS (High Throughput Sampler) The facility at 2117A Everitt Lab (click link for campus map) is equipped with a BD LSR Fortessa analyser. It is very similar in operation to the LSR II cytometers (RAL and ERML Satelite Facilities) but with greater analysing sensitivity.